From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
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From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2008
ISSN: 1940-087X
DOI: 10.3791/831